Crk antibody (pTyr221)

Catalog No. ABIN7271253

The Rabbit Monoclonal anti-Crk antibody (ABIN7271253) specifically detects Crk in WB. The antibody is reactive with Human samples.

Quick Overview for Crk antibody (pTyr221) (ABIN7271253)

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Crk antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Crk Antibody

Binding Specificity: pTyr221

Purpose: Phospho-CRK/CRKII-Y221 Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Phosphorylated Antibodies

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding Y221 of human CRK/CRKII

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: CRK

Background: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],CRKII, p38,Cancer,Cell Biology & Developmental Biology,Cytoskeleton,Cytoskeleton_Actins,Endocrine & Metabolism,ErbB-HER Signaling Pathway,Immunology & Inflammation,Insulin Receptor Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Protein phosphorylation,Signal Transduction,CRK

Molecular Weight: 34kDa

Gene ID: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor