Recombinant SARS-CoV-2 Nucleocapsid antibody (Biotin)

Catalog No. ABIN7273309

The Mouse Monoclonal anti-SARS-CoV-2 Nucleocapsid antibody has been validated for ELISA. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid antibody (Biotin) (ABIN7273309)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type: Recombinant Antibody

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Mouse

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is conjugated to Biotin

Application: ELISA

Clone: AS47

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody (Biotin)

Purpose: Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47)

Characteristics: Recombinant Antibodies produced in HEK293. Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Immunogen: Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (ABIN7271720 and ABIN7273309) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: This antibody can be paired with other Anti-SARS-CoV-2 Nucleocapsid antibodies to detect SARS-CoV-2 Nucleocapsid protein in sandwich ELISA or LFA assay.

Comment:

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Restrictions: For Research Use only

Handling

Format: Powder

Storage: -20 °C

Storage Comment: -20°C

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid protein

Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.