Recombinant SARS-CoV-2 Nucleocapsid antibody

Catalog No. ABIN7271744

The Mouse Chimeric anti-SARS-CoV-2 Nucleocapsid antibody has been validated for ELISA. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid antibody (ABIN7271744)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type: Recombinant Antibody

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Mouse

Clonality: Chimeric

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA

Grade: MALS verified

Clone: AM223

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Purpose: Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) (MALS verified)

Specificity: This product can recognize SARS-CoV-2 Nucleocapsid protein. Cross-reactivity with Nucleocapsid protein of other coronaviruses has not been tested.

Characteristics: Recombinant Antibodies produced in CHO. Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody recombinantly expressed from CHO cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Nucleocapsid Protein. This chimeric antibody is purified by Protein A affinity chromatography. As verified by binding test with N-NTD and N-CTD protein, this antibody can only bind to N-CTD (AA Ser 255 - Pro 364). ELISA test validated that this antibody can potently recognize the N protein variant with mutation located within CTD. It can also bind multiple N protein variants with similar affinity as compared to the wild type N protein.

Immunogen: Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody recombinantly expressed from CHO cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Nucleocapsid Protein. This chimeric antibody is purified by Protein A affinity chromatography. As verified by binding test with N-NTD ABIN7013108 and ABIN7013321) and N-CTD ABIN6992431 and ABIN7013323) protein, this antibody can only bind to N-CTD (AA Ser 255 - Pro 364). ELISA test validated that this antibody can potently recognize the N protein variant with mutation located within CTD ). It can also bind multiple N protein variants with similar affinity as compared to the wild type N protein ABIN6952454 and ABIN6952460).

Isotype: IgG1

Application Details

Application Notes: This antibody can be paired with other Anti-SARS-CoV-2 nucleocapsid antibodies ABIN7271743 and ABIN7273332) to detect SARS-CoV-2 nucleocapsid protein in sandwich ELISA or LFA assay.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -80 °C

Storage Comment: -70°C

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid protein

Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.