NOS1 antibody (Atto 488)

Catalog No. ABIN7272050

The Mouse Monoclonal anti-NOS1 antibody has been validated for WB, IHC, ELISA, ICC and IF. It is suitable to detect NOS1 in samples from Human.

Quick Overview for NOS1 antibody (Atto 488) (ABIN7272050)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: J23

Product Details anti-NOS1 Antibody (Atto 488)

Purpose: Mouse Anti-Human NOS1 Monoclonal IgG2a

Specificity: Ubiquitously expressed, detected in skeletal muscle and brain, also in testis, lung and kidney.,Detects ~160.97 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Partial Human Recombinant NOS1 Protein

Isotype: IgG2a

Application Details

Application Notes: WB (1:1000), optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution was sufficient for detection of NOS1/Nnos in 7.5 ug of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1

Background: NNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. There are two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II)., cellular localisation: Cell Membrane

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction