Recombinant SARS-CoV-2 Nucleocapsid antibody (Alexa Fluor 647)

Catalog No. ABIN7272861

The Alpaca Monoclonal anti-SARS-CoV-2 Nucleocapsid antibody has been validated for IF, IHC and ICC. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid antibody (Alexa Fluor 647) (ABIN7272861)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Alpaca

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is conjugated to Alexa Fluor 647

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 2A7

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody (Alexa Fluor 647)

Expression System: E.coli

Specificity: Nucleocapsid from SARS-CoV-2 and SARS-CoV-1

Purification: Produced in: E.coli

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Application Details

Application Notes: IF: 1:500 (Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.)

Comment:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: Nucleocapsid from SARS-CoV-2

Background: The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.