Recombinant mEos antibody (DBCO)

Catalog No. ABIN7272889

The DBCO-conjugated Alpaca Monoclonal anti-mEos antibody (Clone 1E8) (ABIN7272889) specifically detects mEos in ICC and IF. The antibody is reactive with Lobophyllia hemprichii samples.

Quick Overview for Recombinant mEos antibody (DBCO) (ABIN7272889)

Target: mEos

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Lobophyllia hemprichii

Host: Alpaca

Clonality: Monoclonal

Conjugate: DBCO

Application: Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1E8

Product Details

Expression System: E.coli

Specificity: Recognizes mEOS and mEOS derivatives

Purification: Produced in: E.coli

Immunogen: sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details

Target: mEos

Background: In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.