Recombinant mEos antibody (DBCO)
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- Target
- mEos
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Lobophyllia hemprichii
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Host
-
Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- DBCO
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Application
- Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Recognizes mEOS and mEOS derivatives
- Purification
- Produced in: E.coli
- Immunogen
- sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
- Clone
- 1E8
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- Application Notes
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- Target
- mEos
- Background
- In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.
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