Recombinant RFP antibody (Biotin)

Catalog No. ABIN7272902

This Alpaca Monoclonal antibody specifically detects RFP in IF, ICC and FACS. It exhibits reactivity toward Discosoma.

Quick Overview for Recombinant RFP antibody (Biotin) (ABIN7272902)

Target: RFP (Red Fluorescent Protein (RFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Discosoma

Host: Alpaca

Clonality: Monoclonal

Conjugate: This RFP antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2B12

Product Details anti-RFP Antibody (Biotin)

Expression System: E.coli

Specificity: MRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange

Purification: Produced in: E.coli

Immunogen: Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: mRFP

Background: One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.