Recombinant ALFA-Tag antibody (Alexa Fluor 647)

Catalog No. ABIN7272960

The Alpaca Monoclonal anti- antibody has been validated for WB, FACS, ICC, IF and IHC. It is suitable to detect in samples from .

Quick Overview for Recombinant ALFA-Tag antibody (Alexa Fluor 647) (ABIN7272960)

Target: ALFA-Tag

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Host: Alpaca

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: 1G5

Product Details

Expression System: HEK-293 Cells

Sequence: SRLEEELRRRLTE

Specificity: ALFA-tag

Purification: Produced in: HEK

Immunogen: Single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag: the ALFA tag.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: 1:500 (corresponding to 5 nM final concentration). ALFA tag and the companion FluoTags-X2 anti-ALFA and ALFA-Catcher are the perfect choices for fluorescent-based experiments that want to be combined with biochemical purifications, mass-spectrometry, or any other high-end application.

Comment:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: ALFA-Tag

Target Type: Tag

Background: Owing to the small size of our FluoTags and the short ALFA-tag (15 amino acids; PSRLEEELRRRLTEP), the distance between the target epitope and each fluorophore is below 3 nm. Proteins of interest can be tagged with our ALFA-tag at the N-termini, C-termini, or in-between domains and they can also be immunoprecipitated or natively purified with complexes or partner binders using our series of ALFA-Catcher resins with physiological elution capability.