SARS-CoV-2 NSP1 antibody

Catalog No. ABIN7272996

The Goat Polyclonal anti-SARS-CoV-2 NSP1 antibody is suitable to detect SARS-CoV-2 NSP1 in samples from SARS Coronavirus-2 (SARS-CoV-2). It has been validated for WB.

Quick Overview for SARS-CoV-2 NSP1 antibody (ABIN7272996)

Target: SARS-CoV-2 NSP1 (SARS-CoV-2 Host Translation Inhibitor Nsp1 (SARS-CoV-2 NSP1))

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Goat

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 NSP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SARS-CoV-2 NSP1 Antibody

Purpose: anti-NSP1 (SARS-CoV-2)

Sequence: MESLVPGFNE KTHVQLSLPV LQVRDVLVRG FGDSVEEVLS EARQHLKDGT CGLVEVEKGV LPQLEQPYVF IKRSDARTAP HGHVMVELVA ELEGIQYGRS GETLGVLVPH VGEIPVAYRK VLLRKNGNKG AGGHSYGADL KSFDLGDELG TDPYEDFQEN WNTKHSSGVT RELMRELNGG

Specificity: In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.

Cross-Reactivity (Details): Reacts with SARS-CoV-2 protein

Purification: Epitope affinity purified

Immunogen: Affinity purified recombinant fusion protein Nsp1 of SARS-CoV-2 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for SARS-CoV-2 NSP1

Target: SARS-CoV-2 NSP1 (SARS-CoV-2 Host Translation Inhibitor Nsp1 (SARS-CoV-2 NSP1))

Alternative Name: NSP1 (SARS-CoV-2)

Target Type: Viral Protein

Background:

Host translation inhibitor Nsp1 is part of the multifunctional protein replicase polyprotein 1ab that is involved in the transcription and replication of viral RNA. NSP1 inhibits the host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Therefore, Nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response by suppressing host gene expression.

Host translation inhibitor, Nsp1 SARS Coronavirus-2 antibody.