mEmerald Fluorescent Protein antibody

Catalog No. ABIN7273073

This Goat Polyclonal antibody specifically detects mEmerald Fluorescent Protein in IEM, IF, IHC (fro), IHC (p) and WB. It exhibits reactivity toward Aequorea victoria.

Quick Overview for mEmerald Fluorescent Protein antibody (ABIN7273073)

Target: mEmerald Fluorescent Protein

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Application: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Purpose: anti-mEmerald

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).

Cross-Reactivity (Details): Reacts against Green Fluorescent Protein

Characteristics: Green Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details

Target: mEmerald Fluorescent Protein

Background:

Goat polyclonal antibody to mEmerald (Emerald fluorescent protein). Aquamarine is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. mEmerald is a ~27 kDa protein that is optimally excited at a 487 nm and has a maximum of emission at 509 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

green fluorescent protein antibody.

Molecular Weight: 27 kDa