mOrange Fluorescent Protein antibody

Catalog No. ABIN7273075

This anti- antibody is a Goat Polyclonal antibody detecting in WB, IEM, IF, IHC (fro) and IHC (p). Suitable for Discosoma.

Quick Overview for mOrange Fluorescent Protein antibody (ABIN7273075)

Target: mOrange Fluorescent Protein

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Cross-Reactivity (Details): Reacts against Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Comment:

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Target Details

Target: mOrange Fluorescent Protein

Alternative Name: mOrange

Background: MOrange is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mOrange is a ~27 kDa protein that is optimally excited at a 548 nm and has a maximum of emission at 562 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.