mApple Fluorescent Protein antibody

Catalog No. ABIN7273083

The Goat Polyclonal anti- antibody has been validated for IEM, IF, IHC (fro), IHC (p) and WB. It is suitable to detect in samples from Discosoma.

Quick Overview for mApple Fluorescent Protein antibody (ABIN7273083)

Target: mApple Fluorescent Protein

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Application: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Reacts against Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Comment:

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Target Details

Target: mApple Fluorescent Protein

Background: MApple (Apple fluorescent protein) is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.