mApple Fluorescent Protein antibody

Catalog No. ABIN7273083

The Goat Polyclonal anti-mApple Fluorescent Protein antibody is suitable to detect mApple Fluorescent Protein in samples from Discosoma. It has been validated for IEM, IF, IHC (fro), IHC (p) and WB.

Quick Overview for mApple Fluorescent Protein antibody (ABIN7273083)

Target: mApple Fluorescent Protein

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Application: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Purpose: anti-mApple

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Cross-Reactivity (Details): Reacts against Red Fluorescent Protein

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details

Target: mApple Fluorescent Protein

Background:

Goat polyclonal antibody to mApple (Apple fluorescent protein). mApple is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

red fluorescent protein, Apple antibody.

Molecular Weight: 27 kDa