mCerulean Fluorescent Protein antibody

Catalog No. ABIN7273086

The Goat Polyclonal anti-mCerulean Fluorescent Protein antibody is suitable to detect mCerulean Fluorescent Protein in samples from Aequorea victoria. It has been validated for IEM, IF, IHC (fro), IHC (p) and WB.

Quick Overview for mCerulean Fluorescent Protein antibody (ABIN7273086)

Target: mCerulean Fluorescent Protein

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Application: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Purpose: anti-mCerulean

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).

Cross-Reactivity (Details): Reacts against Green Fluorescent Protein

Characteristics: Green Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details

Target: mCerulean Fluorescent Protein

Background:

Goat polyclonal antibody to mCerulean (Cerulean fluorescent protein). mCerulean is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. mCerulean is a ~27 kDa protein that is optimally excited at a 433 nm and has a maximum of emission at 575 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

green fluorescent protein antibody.

Molecular Weight: 27 kDa