mScarlet antibody

Catalog No. ABIN7273089

The Goat Polyclonal anti-not_set antibody has been validated for IF, IEM, IHC (fro), IHC (p) and WB. It is suitable to detect not_set in samples from Discosoma.

Quick Overview for mScarlet antibody (ABIN7273089)

Target: mScarlet

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Reacts against Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Comment:

In 293HEK cells transfected with cds plasmid detects a band of 26.5 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Target Details

Target: mScarlet

Background: MScarlet (Scarlet fluorescent protein) is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mScarlet is a ~26.5 kDa protein that is optimally excited at a 569 nm and has a maximum of emission at 594 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.