mScarlet antibody
Quick Overview for mScarlet antibody (ABIN7273089)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Purpose
- anti-mScarlet
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Specificity
- In 293HEK cells transfected with cds plasmid detects a band of 26.5 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
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Cross-Reactivity (Details)
- Reacts against Red Fluorescent Protein
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Characteristics
- Red Fluorescent Protein
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Purification
- Epitope affinity purified
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Immunogen
- Purified recombinant fluorescent protein produced in E. coli.
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Isotype
- IgG
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anti-mScarlet antibody (Atto 488)
Reactivity: Dictyosoma IF, FACS, ICC Host: Alpaca Monoclonal 2B12 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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Application Notes
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
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Restrictions
- For Research Use only
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Concentration
- 3 mg/mL
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Buffer
- PBS, 20 % glycerol and 0.05 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- The antibody solution should be gently mixed before use.
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Storage
- 4 °C,-20 °C
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Storage Comment
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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- mScarlet
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Background
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Goat polyclonal antibody to mScarlet (Scarlet fluorescent protein). mScarlet is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mScarlet is a ~26.5 kDa protein that is optimally excited at a 569 nm and has a maximum of emission at 594 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
red fluorescent protein antibody.
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Molecular Weight
- 26.5 kDa
Target
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