mRuby antibody

Catalog No. ABIN7273093

This anti- antibody is a Goat Polyclonal antibody detecting in IF, IEM, IHC (fro), IHC (p) and WB. Suitable for Entacmaea quadricolor.

Quick Overview for mRuby antibody (ABIN7273093)

Target: mRuby

Reactivity: Entacmaea quadricolor

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Reacts against Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Comment:

In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Target Details

Target: mRuby

Background: MRuby (Red fluorescent protein) is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mRuby is a ~26 kDa protein that is optimally excited at a 558 nm and has a maximum of emission at 605 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.