Recombinant mRuby antibody (DBCO)

Catalog No. ABIN7272895

The DBCO-conjugated Alpaca Monoclonal anti-mRuby antibody (Clone 1F8) (ABIN7272895) specifically detects mRuby in IF and ICC. The antibody is reactive with Entacmaea quadricolor samples.

Quick Overview for Recombinant mRuby antibody (DBCO) (ABIN7272895)

Target: mRuby

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Entacmaea quadricolor

Host: Alpaca

Clonality: Monoclonal

Conjugate: DBCO

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1F8

Product Details

Expression System: E.coli

Specificity: Recognizes most common mRuby derivatives

Purification: Produced in: E.coli

Immunogen: sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricolor.

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details

Target: mRuby

Background: MRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.