tdTomato antibody

Catalog No. ABIN7273105

The Goat Polyclonal anti-tdTomato antibody (ABIN7273105) specifically detects tdTomato in IF, IHC (fro), WB, IEM and IHC (p). The antibody is reactive with Discosoma and Tomato samples.

Quick Overview for tdTomato antibody (ABIN7273105)

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reactivity: Discosoma, Tomato

Host: Goat

Clonality: Polyclonal

Conjugate: This tdTomato antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-tdTomato Antibody

Purpose: anti-tdTomato

Sequence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Cross-Reactivity (Details): Reacts against tdTomato, mCherry, RFP

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: preservatives

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Alternative Name: tdTomato

Background:

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It is a ~54 kDa protein that is optimally excited at a 554 nm and has a maximum of emission at 581 nm. It´s brightness and emission wavelength, makes it ideal for live animal research.

red fluorescent protein antibody.

Molecular Weight: 54 kDa