tdTomato antibody

Catalog No. ABIN6254170

This Goat Polyclonal antibody specifically detects tdTomato in IF, IHC (fro), WB, IEM and IHC (p). It exhibits reactivity toward Tag. It has been mentioned in 12+ publications

Quick Overview for tdTomato antibody (ABIN6254170)

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reactivity: Tag

Host: Goat

Clonality: Polyclonal

Conjugate: This tdTomato antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-tdTomato Antibody

Purpose: anti-tdTomato

Sequence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Cross-Reactivity (Details): tdTomato, mCherry, RFP

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-tdTomato antibody (ABIN6254170)

Saavedra, Wallace, Freudenrich, Mall, Mundy, Davila, Shafer, Wernig, Haag: "Comparison of Acute Effects of Neurotoxic Compounds on Network Activity in Human and Rodent Neural Cultures." in: Toxicological sciences : an official journal of the Society of Toxicology, (2021) (PubMed).

Cadwell, Scala, Fahey, Kobak, Mulherkar, Sinz, Papadopoulos, Tan, Johnsson, Hartmanis, Li, Cotton, Tolias, Sandberg, Berens, Jiang, Tolias: "Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex." in: eLife, Vol. 9, (2020) (PubMed).

Unnersjö-Jess, Scott, Sevilla, Patrakka, Blom, Brismar: "Confocal super-resolution imaging of the glomerular filtration barrier enabled by tissue expansion." in: Kidney international, Vol. 93, Issue 4, pp. 1008-1013, (2019) (PubMed).

Park, Choi, Park, Sim, Park, Kang, Yim, Lee, Kim, Pac, Rhee, Lee, Lee, Lee, Kim: "Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio." in: Advanced science (Weinheim, Baden-Wurttemberg, Germany), Vol. 6, Issue 22, pp. 1901673, (2019) (PubMed).

Niquille, Limoni, Markopoulos, Cadilhac, Prados, Holtmaat, Dayer: "Neurogliaform cortical interneurons derive from cells in the preoptic area." in: eLife, Vol. 7, (2019) (PubMed).

Tai, Gallo, Wang, Yu, Van Aelst: "Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM." in: Neuron, Vol. 102, Issue 2, pp. 358-372.e9, (2019) (PubMed).

Ishibashi, Shimizu, Nakata, Fujii, Suzuki, Kawamoto, Anzai, Kuno, Nagata, Ito, Murano, Mizutani, Oshima, Tsuchiya, Nakamura, Watanabe, Okamoto: "Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium." in: Stem cell reports, Vol. 10, Issue 1, pp. 27-42, (2018) (PubMed).

Paolino, Fenlon, Kozulin, Richards, Suárez: "Multiple events of gene manipulation via in pouch electroporation in a marsupial model of mammalian forebrain development." in: Journal of neuroscience methods, Vol. 293, pp. 45-52, (2018) (PubMed).

Hurni, Kolodziejczak, Tomasello, Badia, Jacobshagen, Prados, Dayer: "Transient Cell-intrinsic Activity Regulates the Migration and Laminar Positioning of Cortical Projection Neurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 5, pp. 3052-3063, (2018) (PubMed).

Weider, Starost, Groll, Küspert, Sock, Wedel, Fröb, Schmitt, Baroti, Hartwig, Hillgärtner, Piefke, Fadler, Ehrlich, Ehlert, Stehling, Albrecht, Jabali, Schöler, Winkler, Kuhlmann, Wegner: "Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning." in: Nature communications, Vol. 9, Issue 1, pp. 899, (2018) (PubMed).

Anzo, Sekine, Makihara, Chao, Miura, Chihara: "Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila." in: Genes & development, Vol. 31, Issue 10, pp. 1054-1065, (2017) (PubMed).

Beattie, Postiglione, Burnett, Laukoter, Streicher, Pauler, Xiao, Klezovitch, Vasioukhin, Ghashghaei, Hippenmeyer: "Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells." in: Neuron, Vol. 94, Issue 3, pp. 517-533.e3, (2017) (PubMed).

Target Details for tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Alternative Name: tdTomato

Background:

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.

Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody.