MERS-Coronavirus Spike antibody (AA 726-1296)

Catalog No. ABIN7383858

The Mouse Monoclonal anti-MERS-Coronavirus Spike antibody has been validated for WB. It is suitable to detect MERS-Coronavirus Spike in samples from Middle East Respiratory Syndrome Coronavirus (MERS-CoV).

Quick Overview for MERS-Coronavirus Spike antibody (AA 726-1296) (ABIN7383858)

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Reactivity: Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This MERS-Coronavirus Spike antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MERS-Coronavirus Spike Antibody

Binding Specificity: AA 726-1296

Specificity: Anti-MERS-CoV Spike Protein S2 Monoclonal Antibody

Purification: Protein A Affinity

Immunogen: Recombinant MERS-CoV Spike/S2 Protein (S2 Subunit, aa 726-1296, His Tag), ABIN7198819

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-1:5000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 0.2 μm filtered solution in PBS

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for MERS-Coronavirus Spike

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Alternative Name: MERS-CoV Spike Protein S2

Background: Coronavirus s1,coronavirus s2,coronavirus spike,cov spike,ncov RBD,ncov s1,ncov s2,ncov spike,RBD,S,s1,Spike RBD,The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.