Strep Tag II antibody

Catalog No. ABIN7384516

The Mouse Monoclonal anti-Strep Tag II antibody (Clone 4H10) (ABIN7384516) specifically detects Strep Tag II in WB and IF. The antibody is reactive with Various Species samples.

Quick Overview for Strep Tag II antibody (ABIN7384516)

Target: Strep Tag II

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 4H10

Product Details

Purification: Protein A/G Purified

Immunogen: Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-2000, IP: 3 μg/sample, IF: 1:100-200

Restrictions: For Research Use only

Handling

Concentration: 4.5 mg/mL

Buffer: PBS with 0.1 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details

Target: Strep Tag II

Alternative Name: Strep-Tag II

Target Type: Tag

Background: Strep Tag II, Strep II,The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution.