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beta Actin antibody

ACTB Reactivity: Human, Mouse, Zebrafish (Danio rerio) WB, IF Host: Mouse Monoclonal 2B2 unconjugated

Catalog No. ABIN7384521

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Reactivity
180

130

111

55

42

42

37

27

24

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Human, Mouse, Zebrafish (Danio rerio)
Host
114

82

5

1

1
Mouse
Compatible Secondaries
Clonality
113

88
Monoclonal
Conjugate
129

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
170

76

53

48

33

33

26

21

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

Purification

Protein A/G Purified

Immunogen

Recombinant human Beta actin protein

Clone

2B2

Isotype

IgG2a

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anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian, Donkey WB, IF Host: Goat Polyclonal unconjugated

Application Notes

WB: 1:5000-10000, IP: 3 μg/sample, IF: 1:100-200

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

PBS with 0.1 % sodium azide, 50 % glycerol, pH 7.4

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C. Avoid freeze / thaw cycles.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

beta actin (ACTB Products)

Background

ACTB, Actin beta, Actx, Actin cytoplasmic 1, b actin, A26C1A,Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Molecular Weight

Observed_MW: 43kDa
Calculated_MW: 43kDa

Gene ID

57935

UniProt

Q7ZVF9

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction