PLAP antibody

Catalog No. ABIN7448143

The Mouse Monoclonal anti-PLAP antibody has been validated for WB and IHC (f). It is suitable to detect PLAP in samples from Human.

Quick Overview for PLAP antibody (ABIN7448143)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RALP-870

Product Details anti-PLAP Antibody

Specificity: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Cross-Reactivity (Details): Human.

Purification: 200 µg/mL of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ALPP protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: JEG-3 or HepG2 cells. Human placenta or seminoma.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALPP

Background: Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP1,Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)
Cellular localisation: Cytoplasmic. Cell surface.

Molecular Weight: 70kDa

Gene ID: 250, 284255

UniProt: P05187