RBBP5 antibody (C-Term)

Catalog No. ABIN7449051

This anti-RBBP5 antibody is a Rabbit Polyclonal antibody detecting RBBP5 in WB, IHC, IP, ChIP, ICC and ChIP-seq. Suitable for Human.

Quick Overview for RBBP5 antibody (C-Term) (ABIN7449051)

Target: RBBP5 (Retinoblastoma Binding Protein 5 (RBBP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBBP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-RBBP5 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-RbBP5 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 500 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

ICC: 1:250 - 1:1,000

WB: 1:10,000 - 1:25,000

ChIP: 1 - 3 μg as per Dou et al., Nat Struct Mol Biol 13 (8):713-719, 2006. Previous lots of this antibody have performed in this application.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RBBP5

Target: RBBP5 (Retinoblastoma Binding Protein 5 (RBBP5))

Alternative Name: RbBP5

Background: Background: Retinoblastoma binding protein 5 (RbBP5) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. RbBP5 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, ASH2L,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX. RbBP5 is also one of many proteins that bind the Retinoblastoma tumor suppressor protein, pRb.

Gene ID: 5929

NCBI Accession: NP_005048

UniProt: Q15291