CD45RO antibody

Catalog No. ABIN7449553

This Mouse Monoclonal antibody specifically detects CD45RO in IHC, WB, IF (p), ICC and mIF. It exhibits reactivity toward Human.

Quick Overview for CD45RO antibody (ABIN7449553)

Target: CD45RO

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RO antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Multiplex Immunofluorescence (mIF)

Clone: UCHL-1

Product Details anti-CD45RO Antibody

Purpose: Mouse anti-CD45RO Monoclonal Antibody, Purified [UCHL-1]

Immunogen: Cultured T-cells from an IL-2-dependent T-cell line (CA1)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

ICC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 05:10:00

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD45RO

Target: CD45RO

Alternative Name: CD45RO

Background: Background: CD45RO is also known as protein tyrosine phosphatase receptor type C (PTPRC). It is a protein tyrosine phosphatase required for T-cell activation through the antigen receptor. CD45RO acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45RO recruits and dephosphorylates SKAP1 and FYN. It also dephosphorylates LYN, and thereby modulates LYN activity (By similarity)[taken from UniProtKB (Entry: P08575)].

Gene ID: 5788

NCBI Accession: NP_002829

UniProt: P08575