CD45RO antibody

Catalog No. ABIN7449553

This Mouse Monoclonal antibody specifically detects CD45RO in FACS, IHC, WB, IF (p), ICC and mIF. It exhibits reactivity toward Human.

Quick Overview for CD45RO antibody (ABIN7449553)

Target: CD45RO

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RO antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Multiplex Immunofluorescence (mIF)

Clone: UCHL-1

Product Details anti-CD45RO Antibody

Purpose: Mouse anti-CD45RO Monoclonal Antibody Purified [UCHL-1]

Purification: Purified

Immunogen: Cultured T-cells from an IL-2-dependent T-cell line (CA1)

Isotype: IgG2a

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

ICC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 05:10:00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 50 μg/mL

Buffer: Phosphate Buffered Saline (PBS) containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for CD45RO

Target: CD45RO

Alternative Name: CD45RO

Background: CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08575].

Gene ID: 5788

UniProt: P08575