gamma H2AX antibody (Ser140)

Catalog No. ABIN7449557

The Rabbit Polyclonal anti-gamma H2AX antibody has been validated for WB, IHC and IF/ICC. It is suitable to detect gamma H2AX in samples from Human and Mouse.

Quick Overview for gamma H2AX antibody (Ser140) (ABIN7449557)

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This gamma H2AX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-gamma H2AX Antibody

Binding Specificity: Ser140

Purpose: Rabbit anti-gamma-H2AX Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: surrounding serine 140

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for gamma H2AX

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Alternative Name: gamma-H2AX

Background: Background: H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.

Gene ID: 3014

NCBI Accession: NP_002096

UniProt: P16104