Recombinant CD8 alpha antibody (AA 185-235)

Catalog No. ABIN7449561

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Quick Overview for Recombinant CD8 alpha antibody (AA 185-235) (ABIN7449561)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Multiplex Immunofluorescence (mIF)

Clone: BLR044F

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 185-235

Purpose: Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody [BLR044F]

Immunogen: between AA 185 and 235 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 250 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8 alpha

Background: Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].

Gene ID: 925

NCBI Accession: NP_001759

UniProt: P01732

Pathways: TCR Signaling