SHARPIN antibody (AA 25-75)

Catalog No. ABIN7450727

The Rabbit Polyclonal anti-SHARPIN antibody has been validated for WB and IP. It is suitable to detect SHARPIN in samples from Human.

Quick Overview for SHARPIN antibody (AA 25-75) (ABIN7450727)

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHARPIN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SHARPIN Antibody

Binding Specificity: AA 25-75

Purpose: Rabbit anti-SHARPIN Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: between AA 25 and 75

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SHARPIN

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Alternative Name: SHARPIN

Background: Background: Sharpin (Shank-associated RH domain-interacting protein) is a component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways [taken from the Universal Protein Resource (UniProt) Q9H0F6].

Gene ID: 81858

NCBI Accession: NP_112236

UniProt: Q9H0F6