TFEB antibody (AA 426-476)

Catalog No. ABIN7450814

The Rabbit Polyclonal anti-TFEB antibody has been validated for WB and IP. It is suitable to detect TFEB in samples from Human and Mouse.

Quick Overview for TFEB antibody (AA 426-476) (ABIN7450814)

Target: TFEB (Transcription Factor EB (TFEB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFEB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-TFEB Antibody

Binding Specificity: AA 426-476

Purpose: Rabbit anti-TFEB Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 426 and 476

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TFEB

Target: TFEB (Transcription Factor EB (TFEB))

Alternative Name: TFEB

Background: Background: Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) P19484].

Gene ID: 7942

NCBI Accession: NP_009093

UniProt: P19484