Topoisomerase I antibody (AA 715-765)

Catalog No. ABIN7450832

The Rabbit Polyclonal anti-Topoisomerase I antibody has been validated for WB and IP. It is suitable to detect Topoisomerase I in samples from Human and Mouse.

Quick Overview for Topoisomerase I antibody (AA 715-765) (ABIN7450832)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Topoisomerase I Antibody

Binding Specificity: AA 715-765

Purpose: Rabbit anti-TOP1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 715 and 765

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background: Background: Topoisomerase I (TOPI) functions as an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA [taken from NCBI Entrez Gene (GeneID: 7150)].

Gene ID: 7150

NCBI Accession: NP_003277

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance