NSF antibody (AA 500-550)

Catalog No. ABIN7451135

The Rabbit Polyclonal anti-NSF antibody has been validated for IP. It is suitable to detect NSF in samples from Human.

Quick Overview for NSF antibody (AA 500-550) (ABIN7451135)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-NSF Antibody

Binding Specificity: AA 500-550

Purpose: Rabbit anti-NSF Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Orangutan

Purification: Affinity Purified

Immunogen: Between AA 500 and 550

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7451135 has not performed satisfactorily when used for WB of NSF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NSF.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background: Background: N-ethylmaleimide-sensitive fusion protein (NSF) is required for vesicle-mediated transport. It catalyzes the fusion of transport vesicles within the Golgi cisternae. It is also required for transport from the endoplasmic reticulum to the Golgi stack. NSF seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin [taken from the Universal Protein Resource (UniProt) P46459].

Gene ID: 4905

UniProt: P46459