Dynactin 1 antibody (AA 1228-1278)

Catalog No. ABIN7451920

This Rabbit Polyclonal antibody specifically detects Dynactin 1 in WB and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for Dynactin 1 antibody (AA 1228-1278) (ABIN7451920)

Target: Dynactin 1 (DCTN1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Dynactin 1 Antibody

Binding Specificity: AA 1228-1278

Purpose: Rabbit anti-p150glued Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1228 and 1278

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: p150glued

Background: Background: p150glued is the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. The p150glued subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus [taken from NCBI Entrez Gene (Gene ID: 1639)].

Gene ID: 1639

NCBI Accession: NP_004073

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling