DDX5 antibody (AA 550-600)

Catalog No. ABIN7452198

The Rabbit Polyclonal anti-DDX5 antibody (ABIN7452198) specifically detects DDX5 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for DDX5 antibody (AA 550-600) (ABIN7452198)

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-DDX5 Antibody

Binding Specificity: AA 550-600

Purpose: Rabbit anti-DDX5 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Orangutan

Purification: Affinity Purified

Immunogen: between AA 550 and 600

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,500 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DDX5

Background: Background: DDX5 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. In addition to a role involved in RNA splicing, DDX5 has been observed to interact with and act as a transcriptional coactivator for estrogen receptor alpha (ERalpha). DDX5 has also been found to interact with CBP/300, Smad3, and p53 to modulate transcription. DDX5 is closely related to and forms heterodimers with DDX17.

Gene ID: 1655

NCBI Accession: NP_004387

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus