PDPK1 antibody (AA 506-556)

Catalog No. ABIN7452710

The Rabbit Polyclonal anti-PDPK1 antibody has been validated for WB and IP. It is suitable to detect PDPK1 in samples from Human and Mouse.

Quick Overview for PDPK1 antibody (AA 506-556) (ABIN7452710)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 506-556

Purpose: Rabbit anti-PDK1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 506 and 556

Isotype: IgG

Application Details

Application Notes:

IP: 5 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: Background: PDK1 (3-phosphoinositide-dependent kinase-1) is a serine/threonine kinase that contains a PH domain for binding of phosphatidylinositols (PtdIns). PDK1 phosphorylates PKB/AKT in response to agonist stimulation of phosphoinositide 3-kinases (PI3Ks). Stimulation of PI3Ks generates specific inositol lipids that are important for activation of PDK1. PDK1 may also be responsible for phosphorylating PKA, PKC-zeta, RPS6KA1, and RPS6KB1.

Gene ID: 5170

NCBI Accession: NP_002604

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals