SMG1 antibody (AA 1625-1675)

Catalog No. ABIN7452857

The Rabbit Polyclonal anti-SMG1 antibody has been validated for WB. It is suitable to detect SMG1 in samples from Human.

Quick Overview for SMG1 antibody (AA 1625-1675) (ABIN7452857)

Target: SMG1 (pi-3-Kinase-Related Kinase SMG-1 (SMG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMG1 Antibody

Binding Specificity: AA 1625-1675

Purpose: Rabbit anti-SMG1 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 1625 and 1675

Isotype: IgG

Application Details

Application Notes:

IP: Not recommended. A300-394A is recommended.

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SMG1

Target: SMG1 (pi-3-Kinase-Related Kinase SMG-1 (SMG1))

Alternative Name: SMG1

Background: Background: SMG1 is a PIKK (phosphoinositide 3-kinase related kinases) protein kinase that plays a key role in RNA surveillance via nonsense-mediated mRNA decay (NMD). SMG1 phosphorylates Upf1, a factor that possesses RNA helicase activity and is also central to NMD. SMG1 and UPF-1 have been shown to exist in a complex with the translation termination release factors eRF3, and eRF1. This complex has been termed SURF and associates with the exon junction complex (EJC) to function in the recognition of premature termination codons in aberrant RNAs to trigger NMD.

Gene ID: 23049

UniProt: Q96Q15, Q96QV0

Pathways: Inositol Metabolic Process