Recombinant TWIST1 antibody (AA 1-50)

Catalog No. ABIN7453340

The Rabbit Monoclonal anti-TWIST1 antibody has been validated for WB, IP and ICC. It is suitable to detect TWIST1 in samples from Human.

Quick Overview for Recombinant TWIST1 antibody (AA 1-50) (ABIN7453340)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLR156J

Product Details anti-TWIST1 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-TWIST Recombinant Monoclonal Antibody [BLR156J]

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IP: 20 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 250 μg/mL

Buffer: Phosphate Buffered Saline (PBS) containing 0.2 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST1

Background: Background: TWIST1 is a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. TWIST1 forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. TWIST1 may also regulate neural tube closure, limb development and brown fat metabolism. The TWIST1 gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. [taken from NCBI Entrez Gene (Gene ID: 7291)].

Gene ID: 7291

NCBI Accession: NP_000465

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity