Recombinant CD86 antibody (AA 31-245)

Catalog No. ABIN7453375

This Rabbit Monoclonal antibody specifically detects CD86 in FACS, WB, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD86 antibody (AA 31-245) (ABIN7453375)

Target: CD86

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLR029F

Product Details anti-CD86 Antibody

Binding Specificity: AA 31-245

Purpose: Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR029F]

Immunogen: residues 31-245 (ECD)

Isotype: IgG

Application Details

Application Notes:

IP: 6 μL/0.5 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: Background: CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. CD86 is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response [taken from NCBI Entrez Gene (Gene ID: 942)].

Gene ID: 942

NCBI Accession: NP_787058

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation