ILK antibody (AA 402-451)

Catalog No. ABIN7453381

This Rabbit Polyclonal antibody specifically detects ILK in WB, IP and IF/ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for ILK antibody (AA 402-451) (ABIN7453381)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-ILK Antibody

Binding Specificity: AA 402-451

Purpose: Rabbit anti-ILK Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Guinea pig_10141,Orangutan

Purification: Affinity Purified

Immunogen: between AA 402 and 451

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:100 - 1:500. Acetone fixation is recommended. Permeabilization is not recommended. Formaldehyde fixation is not recommended.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK

Background: Background: Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.

Gene ID: 3611

NCBI Accession: NP_004508

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration