DDIT4 antibody (AA 1-50)

Catalog No. ABIN7453391

The Rabbit Polyclonal anti-DDIT4 antibody has been validated for WB, IP and IF/ICC. It is suitable to detect DDIT4 in samples from Human.

Quick Overview for DDIT4 antibody (AA 1-50) (ABIN7453391)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-DDIT4 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-REDD1 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine,Dog,Horse,Rabbit,Panda,Orangutan,Monkey,Gorilla,Chimpanzee

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IP: 5 - 15 μg/mg lysate

WB: 1:1,000 - 1:4,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: REDD1

Background: Background: REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner. Alternative names for REDD1 include DDIT4, DNA-damage-inducible transcript 4 protein, and RTP801.

Gene ID: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process