NUMA1 antibody (AA 900-950)

Catalog No. ABIN7453400

The Rabbit Polyclonal anti-NUMA1 antibody has been validated for WB, IP and IF (p). It is suitable to detect NUMA1 in samples from Human.

Quick Overview for NUMA1 antibody (AA 900-950) (ABIN7453400)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NUMA1 Antibody

Binding Specificity: AA 900-950

Purpose: Rabbit anti-NUMA Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 900 and 950

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NUMA

Background: Background: The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.

Gene ID: 4926

NCBI Accession: NP_006176

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase