XPO1 antibody (C-Term)

Catalog No. ABIN7453427

The Rabbit Polyclonal anti-XPO1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect XPO1 in samples from Human and Mouse.

Quick Overview for XPO1 antibody (C-Term) (ABIN7453427)

Target: XPO1 (Exportin 1 (XPO1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-XPO1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-CRM1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1025 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: CRM1

Background: Background: Chromosome region maintenance 1 protein homolog (CRM1) is the Ran-GTP-binding nuclear export receptor for proteins bearing a leucine rich nuclear export signal and RNAs. CRM1 also possesses a mitotic function as it localizes to kinetochores, and with Ran-GTP, recruits Ran-GTPase-activating protein (Ran-GAP1) and Ran-binding protein (Ran-BP2) for proper mitotic spindle assembly and chromosome segregation. Additionally, CRM1 is suggested to function as a loading dock of various cell cycle and checkpoint factors such as cyclins, Cdks, p53 and BRCA1, to regulate proper chromosome duplication and genomic stability.

Gene ID: 7514

NCBI Accession: NP_003391

UniProt: O14980

Pathways: M Phase