Geminin antibody (C-Term)

Catalog No. ABIN7453481

The Rabbit Polyclonal anti-Geminin antibody has been validated for WB, IP and IHC (fp). It is suitable to detect Geminin in samples from Human.

Quick Overview for Geminin antibody (C-Term) (ABIN7453481)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Geminin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Geminin Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Geminin Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 160 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: Geminin

Background: Background: Geminin has been identified as a protein that inhibits DNA replication during S, G2, and M phases in association with Cdt1 (chromatin licensing and DNA replication factor). Geminin prevents the binding of the MCM complex onto origins of replication, and its abundance is regulated by ubiquitin ligase activity of the APC/C (anaphase-promoting/cyclosome) complex.

Gene ID: 51053

NCBI Accession: NP_056979

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication