MIS12 antibody (C-Term)
Quick Overview for MIS12 antibody (C-Term) (ABIN7453483)
Target
See all MIS12 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- C-Term
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Purpose
- Rabbit anti-MIS12 Antibody, Affinity Purified
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Predicted Reactivity
- Orangutan
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Purification
- Affinity Purified
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Immunogen
- between AA 150 and C-term
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Isotype
- IgG
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Application Notes
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IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 5 μg/mg lysate
WB: 1:2,000 - 1:10,000
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Restrictions
- For Research Use only
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Concentration
- 1000 μg/mL
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Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Expiry Date
- 12 months
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- MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))
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Alternative Name
- MIS12
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Background
- Background: MIS12 is an inner kinetochore protein found in a complex with Dsn1, Nnf1, and Nsl1 that functions in kinetochore assembly. This MIS12 complex has been demonstrated to be required for proper chromosome alignment and cell cycle progression.
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Gene ID
- 79003
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NCBI Accession
- NP_076944
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UniProt
- Q9H081
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Pathways
- M Phase
Target
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