POLE3 antibody (AA 97-147)

Catalog No. ABIN7453486

This Rabbit Polyclonal antibody specifically detects POLE3 in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for POLE3 antibody (AA 97-147) (ABIN7453486)

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-POLE3 Antibody

Binding Specificity: AA 97-147

Purpose: Rabbit anti-PolE3/p17 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 97 and 147

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: PolE3/p17

Background: Background: PolE3/p17 is a subunit of DNA polymerase epsilon that plays a role in DNA replication and repair. PolE3/p17 contains histone-fold motifs which play a role in protein-protein and protein-DNA interactions. PolE3/p17 interacts with CHRAC1 (chromatin assembly complex 1), ACF1, and ISWI/SNF2H proteins to form the CHRAC ISWI chromatin-remodeling complex.

Gene ID: 54107

NCBI Accession: NP_059139

UniProt: Q9NRF9

Pathways: DNA Damage Repair