USP3 antibody (AA 471-521)

Catalog No. ABIN7453493

The Rabbit Polyclonal anti-USP3 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect USP3 in samples from Human.

Quick Overview for USP3 antibody (AA 471-521) (ABIN7453493)

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-USP3 Antibody

Binding Specificity: AA 471-521

Purpose: Rabbit anti-USP3 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 471 and 521

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7453493 has not performed satisfactorily when used for WB of USP3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of USP3.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for USP3

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Alternative Name: USP3

Background: Background: Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B.

Gene ID: 9960

UniProt: Q9Y6I4