ERF antibody (AA 498-548)

Catalog No. ABIN7453494

This anti-ERF antibody is a Rabbit Polyclonal antibody detecting ERF in WB, IP and IHC (fp). Suitable for Human and Mouse.

Quick Overview for ERF antibody (AA 498-548) (ABIN7453494)

Target: ERF (Ets2 Repressor Factor (ERF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-ERF Antibody

Binding Specificity: AA 498-548

Purpose: Rabbit anti-ERF Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 498 and 548

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7453494 has not performed satisfactorily when used for WB of ERF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ERF.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ERF

Target: ERF (Ets2 Repressor Factor (ERF))

Alternative Name: ERF

Background: Background: Ets2 repressor factor (ERF) is a member of the ETS (e26 transformation specific sequence) family of proteins. ETS family members bear an ETS-domain, a DNA binding domain that recognizes purine-rich sequences. ERF functions as a transcriptional repressor and is an effector of the Erk signaling pathway.

Gene ID: 2077

NCBI Accession: NP_006485

UniProt: P50548