Importin 4 antibody (AA 350-400)

Catalog No. ABIN7453529

The Rabbit Polyclonal anti-Importin 4 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect Importin 4 in samples from Human.

Quick Overview for Importin 4 antibody (AA 350-400) (ABIN7453529)

Target: Importin 4 (IPO4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Importin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Importin 4 Antibody

Binding Specificity: AA 350-400

Purpose: Rabbit anti-Importin 4 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 15 μg/mg lysate

WB: Not recommended. ABIN7453529 has not performed satisfactorily when used for WB of Importin 4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Importin 4.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Importin 4

Target: Importin 4 (IPO4)

Alternative Name: Importin 4

Background: Background: Importin 4 mediates the nuclear import of RPS3A. In vitro, it mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Additionally, along with importin 7, importin 4 has been shown to be involved in nuclear import of HIF-1alpha [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/Q8TEX9].

Gene ID: 79711

NCBI Accession: NP_078934

UniProt: Q8TEX9

Pathways: Protein targeting to Nucleus