MGEA5 antibody (AA 500-550)

Catalog No. ABIN7453549

This Rabbit Polyclonal antibody specifically detects MGEA5 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for MGEA5 antibody (AA 500-550) (ABIN7453549)

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGEA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MGEA5 Antibody

Binding Specificity: AA 500-550

Purpose: Rabbit anti-MGEA5 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 500 and 550

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MGEA5

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Alternative Name: MGEA5

Background: Background: Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 [taken from the Universal Protein Resource (UniProt) O60502].

Gene ID: 10724

NCBI Accession: NP_036347

UniProt: O60502

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process