MED1 antibody (C-Term)

Catalog No. ABIN7453585

This Rabbit Polyclonal antibody specifically detects MED1 in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for MED1 antibody (C-Term) (ABIN7453585)

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MED1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-MED1 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 1525 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1

Background: Background: MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) Q15648].

Gene ID: 5469

NCBI Accession: NP_004765

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha