YBX1 antibody (AA 224-274)

Catalog No. ABIN7453588

This Rabbit Polyclonal antibody specifically detects YBX1 in WB, IHC (fp) and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for YBX1 antibody (AA 224-274) (ABIN7453588)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details anti-YBX1 Antibody

Binding Specificity: AA 224-274

Purpose: Rabbit anti-YB1 Antibody, Affinity Purified

Predicted Reactivity: Rat,X. laevis,Chicken,Bovine,Rabbit

Purification: Affinity Purified

Immunogen: between AA 224 and 274

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: YB1

Background: Background: Y-box-binding protein 1 (YB1) Regulates the transcription of numerous genes. It has been shown to promote separation of DNA strands that contain mismatches or are modified by cisplatin, and shown to have endonucleolytic activity that can introduce nicks or breaks into double-stranded DNA (in vitro). YB1 may play a role in DNA repair. It is a component of the CRD-mediated complex that promotes MYC mRNA stability [taken from the Universal Protein Resource (UniProt) P67809].

Gene ID: 4904

NCBI Accession: NP_004550

UniProt: P67809

Pathways: Regulation of Muscle Cell Differentiation